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Question 6(d)
On what principle is the analytical technique mass spectrometry based?
Step 1
Answer
Mass spectrometry operates on the principle that positive ions (charged particles) can be separated based on their mass-to-charge ratio (m/z). When these ions are generated and then passed through a magnetic field, they experience a force that causes them to move in a specific path. The degree of deflection of these ions in the magnetic field is dependent on their mass; lighter ions are deflected more than heavier ones. This allows for the identification and quantification of different ions based on their respective masses.
Step 2
Answer
The movement of these ions through the magnetic field is crucial as it facilitates their separation. By analyzing the paths taken by the different ions, one can determine their mass and thus gather information about the sample being studied. This analytical technique is widely used in chemistry and biochemistry for characterizing various substances.
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