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Mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are all used in analytical chemistry - Leaving Cert Chemistry - Question b - 2006

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Mass-spectrometry-(MS),-gas-chromatography-(GC),-high-performance-liquid-chromatography-(HPLC)-and-thin-layer-chromatography-(TLC)-are-all-used-in-analytical-chemistry-Leaving Cert Chemistry-Question b-2006.png

Mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are all used in analytical chemist... show full transcript

Worked Solution & Example Answer:Mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are all used in analytical chemistry - Leaving Cert Chemistry - Question b - 2006

Step 1

Give one application of mass spectrometry.

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Answer

One application of mass spectrometry is molecular mass measurement, which is crucial for identifying chemical compounds. This technique is extensively used for analysing organic pollutants in water, allowing for environmental monitoring and regulatory compliance.

Step 2

Given an application of thin-layer chromatography (TLC) in forensic science.

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Answer

In forensic science, thin-layer chromatography (TLC) is employed for drug identification. It assists forensic chemists in separating and identifying components of complex mixtures found at crime scenes, ultimately supporting legal investigations.

Step 3

Give an application of high-performance liquid chromatography (HPLC) in the food industry.

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Answer

High-performance liquid chromatography (HPLC) is utilized in the food industry for growth promoter identification, such as detecting antibiotic residues in meat products. This application ensures food safety and compliance with health regulations.

Step 4

State the principle on which all chromatographic techniques are based.

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Answer

All chromatographic techniques are based on the principle that different substances (components) have different affinities for the stationary and mobile phases. This differential affinity allows for the separation of components as they move through the system.

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